FORMULATION AND DETERMINATION OF TOTAL FLAVONOID CONTENT OF FACE WASH GEL COCOA FRUIT EXTRACT (THEOBROMA CACAO L.) USING UV-VIS SPECTROPHOTOMETRY
DOI:
https://doi.org/10.36858/ipj.v2i1.37Keywords:
cocoa fruit; face wash gel, UV-Vis spectrophotometry; Theobroma cacao L.;Abstract
- Cosmetic products for skin care have recently been very popular among consumers, especially women. One type of face wash preparation that is used every day is face wash gel, this preparation is very suitable for dealing with facial skin problems. Plants or natural ingredients that can be used to formulate face wash gel preparations are cocoa fruit (Theobroma cacao L.) These compounds can be grouped into secondary metabolite compounds which are biomolecules that are used as new discovery materials in the development of herbal cosmetics. This research aims to formulate a face wash gel preparation and determine the total flavonoid content of the face wash gel from cocoa fruit extract (Theobroma cacao L.). This research uses a laboratory experimental research design by extracting cocoa pods using the maceration method with methanol solvent. Cocoa fruit extract face wash gel was formulated with a concentration of 2.5%, 5%, 7.5%, the formula was tested for the physical properties of the preparation, namely organoleptic test, homogeneity test, spreadability test, foam height test, pH test and viscosity test. Then the total flavonoid content of formula 3 face wash gel was determined using UV-Vis spectrophotometry. The results of the physical properties of the face wash gel preparations with concentrations of 2.5%, 5% and 7.5% met the test parameter requirements, while the results of determining the total flavonoid content of the formula 3 face wash gel received a value of 0.0005/mg/QE/g. Test of the physical properties of the face wash gel preparation from cocoa fruit extract (Theobroma cacao L.) meets the requirements and the face wash gel preparation in formula 3 cocoa fruit extract (Theobroma cacao L.) contains total flavonoid levels of 0.0005/mg/QE/ g.
References
Rasydy LOA, Supriyanta J, Novita D. 2019. Formulasi Ekstrak Etanol 96% Daun Sirih Hijau (Piper Betle L.) Dalam Bedak Tabur Anti Jerawat Dan Uji Aktivitas Antiacne Terhadap Staphylococcus Aureus. Journal Farmagazine;6(2):18.
A FZ. 2022. Perbandingan Tepung Sorgum (Sorghum bicolor L. Moench) dengan tepung Beras Merah (Oryza nivara) dan Konsentrasi Biji Edamame (Glycine max (L) Merill) terhadap Karakteristik Foodbar.Skripsi.
Nurkholis ; Rahmawati D; RPR. 2021. Implementasi Sistem Rekording dan Aplikasi Pakan Berbasis Limbah Kulit Edamame Sebagai Upaya Pengembangan Kapasitas Usaha Peternakan Sapi Perah. Sentrinov;7(3):401–11.
Akhita, D P ; Edy, Junaidi ; Septa SW. 2020. Efek Antibakteri Ekstrak Etanol Biji Edamame (Glycine Max (L) Merril) terhadap Bakteri E. Coli. Journal of Agromedicine and Medical Science;6(3):137–42.
Chaleshtori H, Kachoie MA, Mojtaba S, Jazi H. 2017. Antibacterial effects of the methanolic extract of Glycine Max (Soybean). Microbiology Research (Pavia);8:51–4.
Ramadhani S, Elya B, Forestrania RC. 2023. Aktivitas Anti-elastase dan Antioksidan dari Ekstrak Etanol Kayu Bangkal (Nauclea subdita) Korth. Steud. dengan Variasi Metode Ekstraksi. Jurnal Mandala Pharmacon Indonesia;9(2):22843.
Lady D, Handoyo Y, Pranoto ME. 2020. Pengaruh Variasi Suhu Pengeringan Terhadap Pembuatan Simplisia Daun Mimba (Azadirachta Indica). Jurnal Farmasi Tinctura;1(2):4554.
E S, Syamsul ; Nadhila, Ajrina ; Dwi L. 2020. Perbandingan Ekstrak lamur Aquilaria malaccensis dengan Metode Maserasi dan Refluks. Jurnal Riset Kefarmasian Indonesia;2(2).
Agustina W, Handayani D. 2019. Skrining Fitokimia dan Aktivitas Antioksidan Beberapa Fraksi dari Kulit Batang Jarak (Ricinus communis L.). Jurnal Pendidikan dan Ilmu Kimia;1(2):117–22.
Kursia S, Lebang JS, Taebe B, Burhan A, Wa OR. 2019. Antibacterial Activity Test of Ethylacetate Extract of Green Betel Leaf (Piper betle L.) towards Staphylococcus epidermidis Bact. IJPST;3.
Purwaningtiyas N. 2023. Perbandingan Metode Ekstraksi Sokletasi dan UAE terhadap Aktivitas Antijamur Candida albicans pada Ekstrak Biji Coklat (Theobroma cacao L.). Skripsi.
Septia, D N ; Henri ; Occa, Roanisca ; Mahardika GM. 2020. Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-sapu (Baeckea frutescens L.). Journal of Tropical Biology;8(3):178–85.
Berliana H, Pujiyanto S. 2020. Analisis Efektivitas Probiotik di Dalam Produk Kecantikan sebagai Antibakteri terhadap Bakteri Staphylococcus epidermidis. Berkala Bioteknologi;3(2).
Lestari G, Noptahariza R, Rahmadina N, fatah. 2020. Uji Aktivitas Antibakteri Formulasi Sabun Cair Ekstrak Kulit Buah Durian (Durio zibethinus L.) terhadap Bakteri Staphylococcus aureus. Cendekia Journal Pharmaceutical;4(2):95–101.
Fitriyanti, Abdurrazaq, Nazarudin M. 2019. Uji Efektivitas Antibakteri Ekstrak Etil Asetat Bawang Dayak (Eleutherine palmifolia Merr) terhadap Staphylococcus aureus dengan Metode Sumuran. Jurnal Ilmiah Manuntung;5(2):174–82.
Hasanah R. 2023. Uji Aktivitas Antibakteri Etil Asetat Kulit Pisang Tanduk (Musa acuminata x Musa balbisiana) Sebagai Antibakteri Propionibacterium acnes. Skripsi.
Kusuma AE, Dwi D. 2022. Pengaruh Jumlah Pelarut terhadap Rendemen Ekstrak Daun Katuk (Sauropus androgynus L. Merr). Jurnal Farmasi Sains dan Obat Tradisional;1:125–35.
Widyasanti AE, Novira N, Wulandari. 2018. Karakteristik Fisikokimia Antosianin Ekstrak Kulit Buah Naga Merah Menggunakan Metode UAE. Jurnal Ilmiah Rekayasa Pertanian dan Biosistem;6(1):27–38.
Khoiriyah N, Pambudi DB, Slamet S, Rahmatullah S. 2022. Phytochemical Screening And Determination Of SPF Value Of Cocoa Fruit Peel Ethanol Extract (Theobroma cacao L.). Urecol;5:665–73.
Senduk TW, Montolalu Lady, Dotulong V. The rendement of boiled water extract of mature leaves of mangrove Sonneratia alba. Jurnal Perikanan Dan Kelautan Tropical;11(1):9.
Kartikasari RD, Ristia IR, Abduh. 2022. UJi Fitokimia Pada Daun Kesum (Polygonum minus Huds.) dari Kalimantan Barat. Jurnal Insan Farmasi Indonesia;35–42.
Hidayah N, Hisan AK, Solikin A, Mustikaningtyas D. 2016. Uji Efektivitas Ekstrak Sargassum muticum Sebagai Alternatif Obat Bisul Akibat Aktivitas Staphylococcus aureus. Journal Prim Education;1(1).
Kusumo DW, Ningrum EK, Hayu C, Makayasa A. 2022. Skrining Fitokimia Senyawa Metabolit Sekunder pada Ekstrak Etanol Bunga Pepaya (Carica papaya L .). Journal Current Pharmaceutical Science;5(2).
Arianta IA, Datu OS. 2022. Uji Toksisitas Ekstrak Etanol Bunga Kamboja Kuning (Plumeria alba L.) dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT). Pharmacon:1707–14.
Emelda, Safitri, Fatmawati. 2021. Aktivitas Inhibisi Ekstrak Etanolik Ulva lactuca terhadap Bakteri Staphylococcus aureus. Pharmaceutical Journal Indonesia;7(1):43–8.
Norhaliza S, Zamzani I, Nor I. 2022. Potensi Ekstrak Daun Salam (Syzygium polyanthum) dengan Metode UAE Sebagai Antibakteri Terhadap Bakteri Shigella dysenteriae dan Salmonella typhi. Jurnal Ilmu Kefarmasian;3(2):94–101.
Zamzani I, Triadisti N. 2019. Antibacterial Activity of Extract and Fraction of Baccaurea macrocarpa Leaf on Escherichia coli and Bacillus cereus. International Summit Science Technology Humanity,551–6.
Kirtanayasa G. 2022. Literatur Review : Aktivitas Antibakteri Beberapa Ekstrak Tanaman Terhadap Bakteri Klebsiella Pneumonia. Gema Agro;27:107–11.
Yanti, Rosmania R. 2020. Perhitungan Jumlah Bakteri di Laboratorium Mikrobiologi Menggunakan Metode Spektrofotometri. Jurnal Penelitian Sains.
Yunita E, Khodijah Z. 2020. Pengaruh Konsentrasi Pelarut Etanol Saat maserasi terhadap Kadar Kuersetin Ekstrak Daun Asam Jawa (Tamarindus indica L.) secara Spektrofotometri UV-Vis. Jurnal Farmasi Indonesia; 1693-3591.
Firas AA. 2017. Antibacterial Activity of Glycine max L. Seeds Using Different Extracts. Journal Education Science;19-4.
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